Exact(16)
Nano-silica was either dispersed in water using sonication or directly added into cement and mixed using a rotary mixer.
Moderate molecular weight cationic polyacrylamide flocculant was added at a dosage of 3 5 ppm, dependent upon harvest culture density, and mixed using a recirculating centrifugal pump.
The prepared NO/THCPSi NPs (0.1 mg/mL) were added to PBS (1 mL), sonicated, and mixed using a test tube shaker.
Individual standard solutions (10 mg L−1) were infused with the syringe pump and mixed using a tee with the LC flow, mobile phase solvent A: 0.1 % NH4OH, H2O and solvent B: 0.1 % NH4OH, MeOH (50 50), (300 μL min−1), before being introduced into the HESI source.
Acetic/nitric reagent (3 mL of a solution consisting of 10 volumes of 80%% acetic acid and 1 volume of concentrated nitric acid) was added to 0.5 g of the sample in a test tube and mixed using a vortex mixer.
Prior to handling, the samples were thawed at room temperature and mixed using a Vortex (Vortex-Genie, Scientific Industries Inc., Bohemia, NY, USA).
Similar(44)
Twenty milligrams of PCSNs was diluted in 50% ethanol with distilled water (total volume, 20 ml) and mixed using an ultrasonic water bath for 30 min. The diluted solution was filtered using a centrifugal device (ultrafree centrifugal filter, Durapore PVDF 0.1 μm, Millipore, Billerica, MA, USA) and a centrifuge with a radius of 15.0 cm (13,000 rpm, 15 min).
About 7 ml of 10% formalin was added to approximately 1 gram of feces and mixed using an applicator stick.
Second, after foaming, the cement powder was incorporated in the liquid foam and further mixed using a sterile spatula, preventing foam disruption, until complete homogenization of the paste.
Media samples were thawed where necessary and then mixed using a vortex mixer, centrifuged for 10 min at 14,000 × g using a micro centrifuge, and the supernatant removed and placed in an HPLC vial for analysis.
CL reagents (luminol and KMnO4) were incorporated by means of a peristaltic pump and were firstly mixed using a three-way connector.
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