The phrase "and methods and then" is not correct and usable in written English as it lacks clarity and coherence.
It may be intended to connect ideas or steps in a process, but it needs to be restructured for better understanding.
Example: "We will discuss the theories and methods, and then we will proceed with the experiments."
Alternatives: "and techniques, followed by" or "and approaches, after that".
The appropriate congressional overseers should be given the opportunity to read the opinion in a classified setting; the administration could redact portions of the memo that deal with sensitive national security information, including sources and methods, and then release the memo to the public.
Mention methods used in the Materials and Methods and then provide the individual test name in the figure legend for each experiment.
The purified ClPDI was heat-treated as described in the Materials and Methods and then analyzed for the residue PDI activity.
Using the SNP data sets from Tongil and its parents, we defined the genomic origins of regions of the Tongil genome by SNP calling (Additional file 3: Figure S1; Additional file 4: Table S3; see also the SNP calling section in the Materials and Methods), and then performed a SEG-Map analysis (Zhao et al. [2010b]) of Tongil (Figure 2).
They were firstly annealed to complementary oligonucleotides to form double-stranded DNA molecules (see material and methods) and then used for the DNA binding assays.
MBP-specific T cells were generated in the presence or absence of PP14·Fcγ1, as described in Materials and Methods, and then restimulated with MBP, this time without PP14·Fcγ1 in order to characterize the developmental fate of the cells.
Next, we tested for differential expression in our comparisons for each exon separately [see Materials and Methods and [ 25], and then used existing gene models to make inferences about differential expression of isoforms.
The course will first provide an introduction to neuroscience and neuroscientific methods, and then will survey prominent studies in the field of neuroeconomics.
We briefly review existing modeling and solution methods and then present four new approaches to this integrated problem.
Meanwhile, the vibrational characteristics of the cup-shaped ultrasonic transducer have been investigated by using the analytical and numerical methods, and then confirmed by the experiment.
Using historical data as input, the Arena software, Version 13, is adopted to simulate both the traditional and proposed methods, and then compare the results for practicability.
