Sentence examples for and methods and subsequently from inspiring English sources

To this end, U2OS cells were synchronized (double thymidine block, see Materials and methods) and subsequently harvested at different time points after release from this cell cycle block.

The δ sequences-based plasmid pUC57-delta1_2-ADHpr-PHO8-CYCt-kanMX harboring PHO8 gene under the control of ADH1 promoter was constructed as described in Materials and Methods and subsequently transformed into S. cerevisiae laboratory strain BY4742 (Table  1).

First, we constructed Du145-Luc2 cells, which were stably transfected with a firefly luciferase gene (see Materials and Methods), and subsequently infected with lentivirus delivering Cyr61 siRNA (Du145-Luc2/Cyr61i), control siRNA Du145-Luc2/Ctrli Du145-Luc2/Ctrliy), or Cyr61, respectively.

3DOM Fe2O3, which was coated with different molar ratios of Co3O4, was successfully synthesized by inversing PS spheres colloidal crystal template and sol-gel methods, and subsequently characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), N2 absorption-desorption isotherms, X-ray diffraction (XRD).

In the following, we list these "average gain" methods, and subsequently quantify their performance in a typical wireless backhaul network.

A mixed assemblage comprised of two known viruses; E. coli bacteriophages lambda and T4, were sorted using flow cytometric methods and subsequently immobilized in an agarose matrix.

The structures of 1 and 2 were solved by direct methods and subsequently completed by Fourier recycling using SHELXTL.

Tumour-associated RNA- and DNA-based markers are typically identified by high-throughput methods and subsequently validated by standard molecular methods such as qPCR.

In our study, the DEGs were firstly selected by the statistical methods and subsequently ranked by the scores S  (S = { s1, s2, …, s n }).

In this approach, a chemical tag is incorporated into the ODN by SPS or by enzymatic methods and subsequently modified in a second step.

The peptide was prepared on a Rink-Amide resin following general SPPS methods and subsequently cleaved from the resin (1 h, 95 2.5 2.5 TFA/TIS/H2O).