The phrase "and methods and generated" is not correct and lacks clarity in written English.
It may be intended to refer to methods that have been generated or developed, but as it stands, it is confusing and incomplete.
Example: "The research focused on various techniques and methods generated during the study."
Alternatives: "and techniques developed" or "and approaches created".
We sequenced the RNA of twelve samples which includes the two parental genotypes and the hybrids exposed to two contrasting treatments (3 genotypes × 2 treatments × 2 replicates) using Illumina platform (described in the Materials and Methods) and generated a pseudo-molecule based on the O. sativa MSU7 cDNA (Nipponbare, a japonica).
We examined this by modeling group coevolution with the simple model of in silico evolution described in the Materials and Methods and generated 10 independent alignments where the size of the coevolving group varied between 0 (no covariation) and 10, and the probability of coevolution was varied in increments between 0 and 0.95.
Of the complexes with unknown structure listed in Figure 3, we selected four cases with two or more high GREMLIN score (≥0.6) contact predictions across the interface that had experimentally determined structures for most of the subunits ('Materials and methods') and generated structural models of the complexes.
To examine the PK parameters of commercially available DCU, we dosed SD rats using 3 mg/kg IV bolus, oral solution and oral suspension administration methods and generated corresponding plasma concentration versus time curves (Fig. 1).
XL participated in the analysis of tasiRNA and natsiRNA using computational methods and generated the miR173-tasiRNAs PPR/ TPR network.
JRK implemented the proposed method and generated experimental results.
DNA was isolated from the remaining sample (see Materials and Methods) and was used to generate a large fosmid library, AD1000 (38,704 clones) (Martin-Cuadrado et al. 2008).
For intervals without these required data, the pressure from both Bowers (1995; Eq. 1) and Eaton (1972 and 1975; Eqs. 2, 4 and 5) methods were generated and compared.
In the present study, we have described a novel peptide, referred to as peptide cA1, designed using the inverted hydropathy, i.e. complementary [antisense] strand DNA method (as described in Introduction and Material and Methods), which generated a cellular response towards the recombinant gp120 protein in cA1 peptide-immunized rabbit.
By various bioinformatics methods ('Materials and methods'), we generated assemblies containing 5953 and 1064 scaffolds for Chromera and Vitrella, respectively.
Using a transient injection approach (see 'Materials and methods'), we generated Tg hcrt ChR2-EYFP) and Tg hcrt ChR2-EYFPvandin which single LC neurons expressed Tg hcrtEGFPalarvaecoded calcinm indicator GCaMP6s.
