The phrase "and methods and after" is not correct and does not convey a clear meaning in written English.
It appears to be an incomplete thought or a fragment that lacks context, making it difficult to determine its intended use.
Example: "We will discuss the techniques and methods and after that, we will analyze the results."
Alternatives: "and techniques following" or "and approaches thereafter".
Cells were plated as described (see Materials and Methods) and after the third day in culture, medium was changed to medium containing 0.1, 1.0 or 10 µM RA; control cultures were supplemented with medium without retinoid.
Luciferase activity obtained by the improved transfection protocol (see Materials and Methods) and after doxycycline induction was equivalent to 7×10 enzyme units per 10 Bro-Tet-On cells, and to 3×10 units per 10 SKM13-Tet-On cells, respectively, what was more than 1000-fold over background (untransfected cells).
The cells were plated into cell culture inserts as described (see Materials and Methods) and grown up to four days after confluence, when they had already formed a 3 4 layered epithelium, and TER values were determined.
We next measured the fraction of variance of the changes in neural state (6-D) that could be explained by rotational activity alone (see 'Materials and methods') and found that this greatly decreased after shuffling.
Patients and methods: Before-after study.
Methods design Before-and-after study.
Data will be collected and analysed using both quantitative and qualitative methods during and immediately after the intervention.
See the full test results tables and methods after the jump.
Materials and methods Four hours after cecal ligation and puncture (CLP), Wistar male rats were randomly allocated to the following groups (n = 8): CLP, CLP + E1 (Esmolol: 1 mg kg−1 h−1), CLP + E5 (Esmolol: 5 mg kg−1 h−1), CLP + E18 (Esmolol: 18 mg kg−1 h−1).
Figure 3 shows the fraction of QDs recovered (see Materials and Methods) after each step.
Patients and methods: A before and after prospective, observational, non-interventional study was conducted in surgical ICU in Caen University Hospital, between November 2014 and April 2017.
