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Single optical sections (upper panel) and maximum projection of the entire Z-stacks (lower panel) of typical signal patterns for the wild type and exJM-deleted FER-GFP.

Z-series were performed with a Z-step of 1 µm and maximum projections of Z planes including the spindle region are displayed.

All parts of the microscope systems were controlled by the software package MetaMorph (version 7; Molecular Devices), which was also used for image processing including the adjustment of brightness and contrast, as well as measurements, quantifications, kymographs, and maximum projections of z-stacks.

Green fluorescence (500 540 nm) was collected in one channel, autofluorescence (580 680 nm) was collected in a second channel and maximum projection images of 20 30 µm tissue slabs were overlaid in order to search visually for microbes within the leaf microstructure.

Three-dimensional projections and maximum projection images of front view, top view, and side view were assessed using the Leica LAS AF software.

10 images per time point were collected, and each image was the maximum projection of four confocal sections of ∼1 μm thickness with 0.5 μm step.

Both the maximum projection of rainfall intensity and the maximum change in future projections are affected by using different reference periods for different frequencies and durations.

(A – H ) Maximum projection of tonoplast localised SNAREs and marker protein NET4A under high auxin conditions.

(F ) SIM maximum projection of (top) wild-type and (bottom) Gravin null MEFs at metaphase.

Leica software (Application Suite 1.8.0) was used to create a maximum projection of the z-stack.

Maximum projection of z-stacked spinning-disk confocal images is shown.