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For the most part, the link between signals and features for a particular classification task is still performed heuristically and manually in the signal processing research community.

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Protein features chosen for mass spectrometric analysis were excised from the gels and manually in-gel digested with trypsin following the protocol defined by Shevchenko [15].

The KEGG pathways, not just cancer pathways, are constructed and curated by research staff at the Kyoto University, who read literature and manually build-in the connections.

Full length sequences for all taxa were aligned with Muscle 3.6 [48] and edited manually in the case of clear errors and to remove gaps.

However, many scientists still have to conduct experiments and manually fill in the gaps between the scaffolded contigs.

The catheter was navigated with the Stereotaxis Niobe II system in the RMN group (n = 107) and guided manually in the CON group (n = 54).

The codon alignment sequences that contain frameshift mutations were checked and edited manually in the software MEGA4 [ 23] if identity is low.

Paralogous sequences were aligned using ClustalW2 at the EMBL-EBI site and checked manually in the sequence alignment editor Se-Al [ http://tree.bio.ed.ac.uk/software/seal/].

For intracerebral inoculation, mice were anaesthetized with isoflurane and injected manually in the left parietal lobe with a 27-gauge, 0.5-inch needle.

Full length sequences for all taxa were aligned with Muscle 3.6 [ 87] and edited manually in the case of clear errors.

The type of surgical treatment registered in DGCD was validated in NPR and checked manually in the medical files of DGCD in case of missing data or operations deviating the expected operation for a given cervical cancer type.

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