Sentence examples for and labeled using a from inspiring English sources

Hybridization of the membrane was performed overnight at 42°C in a hybridization solution with the addition of denatured probe, [P]dCTP-labelled cDNA, corresponding to PINK1 amplified by PCR (primers in table 1) and labeled using a random priming kit (Amersham Biosciences).

DNA fragments for EMSA were obtained by PCR amplification and labeled using a biotin labeling kit (Invitrogen).

Activated regions were identified and labeled using a standard atlas of the rhesus monkey brain [45].

Samples were amplified and labeled using a custom automated version of the RT/IVT protocol and reagents provided by Affymetrix (Santa Clara, CA).

The cells were rinsed in PBS and labeled, using a solution of the label and enzyme solutions from an in situ cell death detection kit, fluorescein (Roche Applied Sciences, Mannheim, Germany), with appropriate controls labeled only with the label solution.

All samples, with the exception of kidney medulla, were amplified and labeled using a custom automated version of a 5 µg RT/IVT protocol and hybridizations to custom Agilent microarrays were performed as described [58].

After DNA extraction, the target rDNA genes were amplified and labeled using an asymmetric PCR; then, the amplicons were hybridized to the oligonucleotide probes present on the chips.

One µg total RNA was linearly amplified and labeled, using an Amino Allyl MessageAmp™ II aRNA amplification kit (Ambion TX, U.S.A).

Total miRNA from 1 × 10 cells was isolated and labeled using an mirVANA™ miRNA Isolation kit and mirVANA™ miRNA labeling kit (Ambion, Austin, TX, USA).. Samples (4 μg) labeled with Cy3/Cy5 were hybridized on miRNA microarrays (CSC-GE-3, chipscreen biosciences, Shenzhen, China).

and labelled using a Nick Translation kit and Spectrum Orange dUTPs (Abbott Molecular Inc., Des Plaines, IL, USA).

DNA was amplified in a multiplex PCR and labelled using a locus-specific single base extension reaction.