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Introns are shown by tilde, and number of nucleotides between primer and intron is presented in bracket.
The rps16 gene and the rps12i346 intron are present in the plastid genomes of many land plants, including Angiopteris and all examined leptosporangiate ferns (Table 2; Figure 4), indicating that they were probably present in the fern common ancestor.
Comparisons among the intron sets of the four known zygnematophycean chloroplast genomes indicate that at least 19 of 21 embryophyte group II introns were present in the last common ancestor of conjugating algae; the two absent introns being trnIGAU-intron and trnVUAC-intron (table 2).
The possible conclusion is that introns were present in the most primitive genes, the very first genes were intron-dense, and that the genes of the smaller intron-poor (eukaryotic parasites and yeast) and intron-less (bacteria) genomes were derived from the intron-rich original genes by loss of introns.
No introns were present in the monogonont sequences.
Additionally, while two group II introns are present in R. salina mtDNA, the H. andersenii mtDNA is devoid of introns (Table 2) [ 24].
It is thus possible that these introns are present in other copies of the other species.
In B. plumosa 13 introns are present in 12 genes, comprising 8.3% (including intronic ORFs) of the total cpDNA (Tables 1 and 4).
The petB and psbA genes each contain one intron, whereas three introns are present in rrl.
2. Nested-Clade Intron Gains Nested-Clade Intron Gains In the case of 61 introns in 57 genes, derived introns are present in only a subset of sampled D. pulex clones.
We also show that not only Group I introns but also Group II introns are present in sponges.
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