In the upper layers near the dorsal surface of the cortex where GFAP+ cells exhibit a higher population density, thicker and less branched processes and more intensive expression of GFAP (also known as interlaminar astrocytes, Nag 2011), GFAP+ astrocytes comprised near half of all FABP7+ cells, with the remainder being NG2+ OPCs (data not shown).
Our previous study showed that the ACCs histological type which presented high metastasis tendency exhibited higher microvessel density levels and more intensive expression of angiogenic related factors [ 3].
In our 96 informative cases of ovarian carcinomas by both IHC and FISH simultaneously, intensive expression of Bmi-1 was detected in all (8/8) ovarian carcinomas that had Bmi-1 amplification.
For statistical analysis, and were counted as low expression of Bmi-1 (Fig. 1A, B and 1C), while and were counted as intensive expression of Bmi-1 (Fig. 1D and 1E).
In our study, we defined that and were counted as low expression of Bmi-1, while and were counted as intensive expression of Bmi-1.
These processes are accompanied by the intensive expression of p21 and Chk1 kinase, a slight increase in B1 cyclin level, degradation of Cdc25C phosphatase, and activation of MAPK and p38 kinases (Zhang et al. 2001; Zhang et al. 2003).
In ovarian carcinomas, significant positive associations were found between intensive expression of Bmi-1 and the tumors ascending histological grade, later pT/pN/pM and FIGO stages (P < 0.05).
FISH results demonstrated that the amplification of Bmi-1 was not detected in any of the ovarian cystadenoma and borderline tumor tissues; but was detected in 8% (8/96) of the informative ovarian carcinomas; in each of the 8 cases with Bmi-1 amplification, intensive expression of Bmi-1 was observed (Fig. 1E and 1F).
A significant increasing frequency of intensive expression of Bmi-1 was observed in cystadenomas, in borderline tumors and in invasive carcinomas (P < 0.01, Chi-Square Test for Trend) The association between Bmi-1 expression in ovarian carcinomas and several known clinico-pathological features was further studied.
It is consistent with the intensive expression of BmRas transcripts after the late stage of 5th instar, the period of adult tissue development.
