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Cells were transformed with 45 µl of the PCR reaction, using the lithium acetate method [49], and integrations on the PNC1 locus were confirmed by PCR.
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More specifically, given the mechanistic similarity between MGE transposition and integration, on the one hand, and insertion of spacers by the CRISPR-Cas system and immunoglobulin gene rearrangement, on the other hand, integrases and transposases appear to be ready-made tools that can be recruited and utilized by adaptive immunity systems.
Introduction to real analysis, including the theory of Lebesgue measure and integration on the line and n-dimensional space and the theory of Fourier series.
Discretization and integration on the basic grid gives (2.27).
This election is meaningful in that it is an election to abolish the privileged class and to lay a cornerstone of the society called "reform". President-elect Moon called it "eradication of deep-rooted evil" and "reform and integration". On the last day of the election campaign, he said, "Give me the strength to overcome the current crisis that the country faces.
Upon transformation and integration on the chromosome, the mutation was verified by sequencing.
Cisco also announced it was launching a $150 million fund to encourage third party developers to build applications, bots and integrations on top of the Spark platform.
Emphasis is on representation and integration of the many diverse data sources, and their presentation for biomedical and pharmaceutical researchers.
Its emphasis is on usability and integration of the best analytic tools.
This paper focuses on the recent progress made on the design and integration of the diagnostic.
Other sessions focused on screening, preparation and integration of the experience into the patients' everyday lives.
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