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Accelerometer data were downloaded and inspected using the manufacturer's software (Actical V2.12, Mini Mitter Co. Bend, OR).
Over 1,000,000 transcript sequences for Arabidopsis, rice and maize and around 90,000 transcript sequences for poplar were downloaded from the PlantGDB database [ 41] and inspected using the SeqClean package downloaded from [ 42].
GD18 and P1 brain structures were stained with H&E as previously reported (Mattan et al., 2010) and inspected using the Axiovision software on a Zeiss Axioskop with an Axiocam.
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Carbon-fibre coupons containing impact damage were manufactured and then inspected using the novel strain-based damage assessment technique and an ultrasonic technique commonly applied in industry.
Multiple alignments were done with MUSCLE 3.8.31 [ 112] and manually inspected using the ED program from the MUST package to remove non-homologous or partial sequences [ 113].
Samples were genotyped using a MassArray4 instrument, and the genotypes were assigned with MassArrayTyper (Version 4 running Typer Analyzer 4.0.22.67) and manually inspected using the MassArray Typer v. 3.3 software.
Predicted SNPs within the PZ, PMP gene cluster and the 77 kbp hypervariable region as well as within the six potential pseudogenes were manually and visually inspected using the BAM files generated from the BWA read mapping in Artemis.
Multiple alignments were performed with MUSCLE v3.8.31 (Edgar 2004) and manually inspected using the ED program from the MUST package (Philippe 1993) to verify that all sequences retrieved at the first step were homologous.
786-O and ACHN cell lines were transfected with miR-646, miR-NC, NOB1-shRNA, control shRNA or non-treated controls and were inspected using the MTT [3- 4,5-dimethyl-2-thiazolyl -2,5-diphenyl-2H tetrazolium bromide] assay.
Furthermore, two additional randomly chosen genes, FGSG_04300 and FGSG_08487, were inspected using the same strategy as described for FGSG_01636, and both lacked the predicted introns in the amplified fragments.
Concentrations were estimated by A260 and mRNA quality inspected using the RNA 6000 Nanochip kit on an Agilent 2100 bioanalyzer.
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CEO of Professional Science Editing for Scientists @ prosciediting.com