Sentence examples for and inserted with the from inspiring English sources

Firstly, the EGFP fragment (700 bp) in the plasmid pBac[3xP3-DsRed, HS-EGFP] [35] was replaced with the 74 bp White intron 2 (X02974) of Drosophila melanogaster and flanking MCSs (PacI, AsiSI, AflII, AarI and NheI in 5'end; SpeI, BspEI, AfeI and StuI in 3' end) as a spacer/linker, cut and inserted with the flanking HpaI and NotI (Fig. 1B).

Adenoviral vector (Adv) and Adenoviral vector inserted with the vpr gene (Adv-Vpr) were kindly provided by Dr L. J. Zhao [17].

The two putative proteins, PP6 and PP7, were probably inserted with the retrotransposon.

Glyn-Jones et al. (2006) compared the effect of surgical approach on migration of Exeter stems, and found that stems inserted with the posterior approach showed statistically significantly greater amounts of retroversion: 1.16° (lateral technique) and 1.94° (posterior technique).

RhPV 5' IRES sequences (in sense or antisense orientation) or other intergenic sequences were amplified and inserted, along with the AcGFP or DsRed ORFs, into the XhoI and StuI sites of the modified pSLfa.

The endoscope was coated with lubricant and inserted into the anus with the mouse in a dorsal supine position.

Furthermore, the pigmented cells situated within the cornea stroma and inserted anteriorly with adhesion to the posterior part of the cornea (Fig.  3d).

One end plugged into the charging post; I took the other end, went to the car, flipped open a little flap and inserted the nozzle with a satisfying click.

Libraries of GFPmut2 were synthesized using the mutagenic method of Fromant et al. [45] and inserted in-frame with the coding region for the DsbA signal peptide.

MO generation: A MO is generated and inserted into the RoI with fixed seed.

As shown in Fig.  2b, the vgb gene has been successfully cloned and inserted into the pBSPPcGm with the corresponding restriction enzyme sites, obtaining the pBSPPcGm- vgb.