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SLIC cloning enables precise and sequence independent engineering and is based on joining vector and insert with 15 25 bp homologies on both DNA ends by homologous recombination.
SLIC cloning enables sequence independent, precise cloning with minimal or no changes in the amino acid sequence of the target protein and is based on homologous recombination of vector and insert with 15 25 bp homologies on both DNA ends.
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H358 cells were engineered and inserted with inducible transactivation vectors.
A Sunday newwpaper often includes several press runs - with some sections printed early and inserted with the remainin parts prior to delivery.
To construct g4HA-TTG1, the whole-genomic region of TTG1 was amplified into two fragments (−2526 to −1; 1–2136), and inserted with a 4HA tag by overlapping PCR.
L. 114 95, § 1010(2)(C)(iii), substituted "90percentt" for "95percentt" and inserted ", with priority given to high-need schools" after "schools served under this part".
To compare the complications and outcomes of Descemet stripping automated endothelial keratoplasty (DSAEK) when the tissue is either folded and inserted with a forceps or inserted using a platform injector device without folding.
The gondolas were filmed in the moat on Caerphilly Castle and inserted with CGI.
Witnesses aren't escorted into the death chamber until the inmate has been prepared, tied down and inserted with IV lines.
In 57 eyes (95%), the graft was folded and inserted with forceps, and in three eyes (5%) it was inserted with a funnel glide.
Migration and invasion assays were performed using 24-well transwell plates and inserts with 8-μm pore size (Corning, Inc., Corning, NY).
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