Sentence examples for and inoculated into a from inspiring English sources

Purpose: This study investigated the ability of chondrocytes grown in culture and inoculated into a newly developed biodegradable sponge to form ectopic cartilage tissue.

Single colonies from the solid medium were selected randomly and inoculated into a test tube containing 3 mL fermentation medium for 96 h fermentation.

A seed medium was prepared and inoculated into a 15-L fermentor containing a 10-L working volume (4 g/L of yeast extract, 10 g/L of malt extract and 25 g/L of glucose).

Fig. 5 Fed-batch biotransformation of FA. C. glutamicum strains FVan (closed symbols) and F (open symbols) were cultivated in BY medium in a flask for 24 h, collected, and inoculated into a bioreactor.

One colony from each flocculent phenotype, as well as from the wild-type, was selected and inoculated into a 50 mL flask, containing 10 mL YPD medium, and incubated for 16 hours at 30°C in a rotary shaker at 250 rpm.

For keratinase production, recombinant B. subtilis strains were cultured overnight at 37 °C and inoculated into a 250-mL shake flask containing fresh LB medium (30 mL) with cell density of OD600 = 0.1, and then incubated in an orbital shaker at 200 rpm and 37 °C for 24 h.

Bacteria were extracted from a deciduous forest soil and inoculated into an aqueous medium.

For long-term chemostats, MC4100TF was grown overnight in T-salts and inoculated into an 80-ml chemostat containing T-salts, 0.2% glucose and 30 μM KH2PO4 as described (Spira and Ferenci 2008).

The spore suspension was harvested from the cultures grown on potato dextrose agar (Wako Pure Chemical Industries, Osaka, Japan) slants, and were inoculated into A-1 medium (Miyauchi et al. 2015).

After digestion, the cell suspension was washed in the Ham's F12 medium (Mediatech) once, and inoculated into flasks in a DMEM/HamF12 (PAA) medium containing 10% NCS (PAA), with 50 g/ml gentamycin sulfate (PAA), 2 g/ml amphotericin B (PAA) and 50 g/ml L-ascorbic acid (PLIVA, Kraków, Poland).

Chlamydomonas sp. JSC4 was precultured in Modified Bold 3 N medium and inoculated into the PBRs at a concentration of 90 to 100 mg L−1.