Sentence examples for and induced with from inspiring English sources

For colony formation assays stable tetracycline inducible cells were serum starved for 48 hours and induced with 1 µg/ml tetracycline (Sigma, St . Louis MO if applicable.

The cells were grown to mid-log phase and induced with 0.5 mmol/L IPTG at 25 °C overnight.

Lanes 5 6 soluble VEGF165 expressed in LBA medium and induced with 0.4 mM IPTG at 25 and 37 °C respectively.

Lanes 5 7 insoluble VEGF165 expressed in LBA medium and induced with 0.4 mM IPTG at 25, 30 and 37 °C respectively.

For imaging studies, the mouse was pre-oxygenated for 5 min with 100% FiO2 and induced with 5% isoflurane at 2 L/min prior to intubation [13].

Importantly, USCs can be obtained via a non-invasive, simple, and low-cost approach and induced with high efficiency to differentiate into three dermal cell lineages.

Bacteria were grown to an OD600 of 0.6 and induced with 1mM IPTG.

The fusion protein was overexpressed at 37°C and induced with 1 mM IPTG.

Cultures were grown to 0.6 OD and induced with IPTG to overexpress proteins for 1 hr at 37°C.

Briefly, DH5α Ε. coli cells were transformed and induced with IPTG (0.5 µM) overnight for 16 hrs.

In subsequent experiments, COS7 cells were starved and induced with EGF, a strong elicitor of mitogenic signalling.