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Cells were seeded in 96-well plates and incubated in a CO2 incubator at 37 °C.
Each membrane disk (φ8 mm, 1 mm thick) was immersed in PBS containing trypsin and incubated in a CO2 incubator.
Flasks were closed with cotton stoppers and incubated in a shaker incubator (26 ± 1 °C, 150 rpm).
Bacteria were cultured in nutrient broth medium (pH 6.8) and incubated in a shaking incubator at 37 °C and 180 rpm.
The tubes were then seeded with 1 mL fresh culture of bacterial strains and incubated in a shaking incubator at 37 °C and for 48 h.
The cells were then seeded into microtiter plates and incubated in a CO2 incubator at 37°C for 24 hrs.
DHE staining was then applied to each tissue section and incubated in a light-protected incubator at 37 °C for 30 min.
The fusion cells were raised in 96-well plates and incubated in a 5% CO2 incubator.
Cells were transferred from cuvettes to pre-warmed culture medium and incubated in a 5% CO2 incubator at 37°C.
HepG2 cells were cultured in 96-well plates at a density of 1 × 104 cells/well and incubated in a 37 °C, 5%% CO2 incubator for 24 h.
Inoculated plates were covered and incubated in a Titramax 1000 vibrational platform incubator (Heidolph Instruments, Germany) at 150 rpm, 28 °C (or 37 °C for E. coli).
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