Laboratory files were downloaded from the NHANES website in October 2012 and included the needed corrections for impurities in some of the previously used analytical standards (Langlois et al. 2012).
The main problems with pyrosequencing are discussed and include the need for expensive biotinylated primers and the inability to accurately detect variants within long homopolymer stretches.
Moreover, the ease of moving data across (i.e. portability) cloud providers' platform mandates data to be in a compatible format [34], and includes the need to securely delete the old storage [36].
Policy recommendations are provided and include the need for skepticism in long-term projections, avoiding picking specific technology winners, and the need for enhanced valuation systems for environmental externalities.
The challenge however is significant and includes the need for a thorough understanding of the aerodynamics involved, as well as the development of the mechanics and wing transmission mechanisms as well as the controller for successful flight".
Knowledge gaps and future research needs are outlined, and include the need for more consistency in study designs, incorporation of reaction product measurements into epidemiological studies conducted in both indoor and ambient settings, and more focused research on the toxicity of SOA, especially SOA of biogenic origin.
Alternative assumptions examining shorter vaccine efficacy durations and including the need for a repeat dose or possible waning in vaccine efficacy were considered in the sensitivity analysis.
Other key challenges to STRIVE are those characteristic of remote environments and include the need to sustain the motivation of clinic staff to participate in STRIVE while addressing the multiple competing health priorities, as well as high staff turnover.
However, more recently consultation with patients, public and NHS staff has worked to define the emotional aspects of positive patient experiences and include the need to feel cared for, safe, confident and in control, being communicated with as an equal, and being treated with honesty, dignity and respect [ 24].
