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The three B-WICH core components, the WSTF, SNF2h and NM1 proteins, are also found at promoters and in the transcribed regions of rRNA genes, and they are required for increased RNA pol I transcription [20].
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The quotes are presented indicating the specific interview (specified in capital letters), which of the informants that spoke in the group (indicated by numbers) and the page number in the transcribed text.
Meaningful segments of data were identified and coded with appropriate labels in the transcribed text.
Glass's Mad Rush (1979) and In the Summer (1993, transcribed by the Chuns in 2014) carry his trademark arpeggiated patterns and ever unfolding melodies, which here take on a silvery, high-violin hue.
All plasmids were linearized with NotI and in vitro transcribed from the minimal SP6 promoter using the mMESSAGE mMACHINE kit (Ambion, Austin, Texas) following the manufacturer's instructions.
The pT7-3×Flag-hCas9 plasmid was linearized with PmeI and in vitro transcribed using the mMESSAGE mMACHINE T7 ULTRA kit (Life Technologies).
Two candidate small interfering RNAs (siRNAs) corresponding to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike gene were designed and in vitro transcribed to explore the possibility of silencing SARS-CoV S gene.
PCR products representing five different B. subtilis DNAs were printed onto the microarrays and in vitro transcribed into RNA.
PCR products representing approximately 40 regions from the M. jannaschii genome ranging from 250 10,000 bps were printed onto the microarrays, and in vitro transcribed into RNA.
We target double strand breaks to specific sites within the genome by injecting mRNA encoding the Cas9 endonuclease and in vitro transcribed synthetic guide RNA into Drosophila embryos.
However, much of the footage broadcast by Four Corners is described, and in some instances transcribed, within the children's commissioner's report which the briefings referenced.
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