Regarding HO-1−/− mice, immunohistochemical analysis demonstrated a small number of astrocytes and microglia in the STR of saline-inoculated HO-1−/− mice that was similar to their wild type controls (Figs. 6A and 6B).
Despite the low genome coverage and the low percentage of genes expressed in AltDE1, the difference between AltDE and AltDE1 in the STR condition is notable.
Also, copper extractions calculated for the bioleaching using mesophilic bacteria were 48.73% and 37.19% in the STR (stirred tank reactor) and the airlift bioreactor, respectively.
In these tests, after 96 h, maximum enzymatic activity values achieved for exo- and endo-PG were 65.2 units (U) per mL and 91.3 U mL−1, in the STR, with similar activity values of 60.6 U mL−1 and 86.2 U mL−1, respectively, being achieved in the airlift bioreactor.
Unlike pyramidal cells in the CA1 and CA3 areas, interneuron somata were α1 subunit immunopositive, along with a dense meshwork of axonal fibers and terminals localized predominantly in the str.
Tyrosine hydroxylase (TH)+ fiber density and TH+ (dopamine-producing) neurons were significantly reduced in the STR and substantia nigra (SN) in Ifnb –/– mice versus WT.
Real-time PCR analysis showed that mRNA levels of p62 are reduced in the STR, CB and CTX, and there were no significant changes in the SN and HIP regions (Fig. 1D).
The soluble medium was used in tests to compare the production of these enzymes in the STR and airlift bioreactor.
In vivo chronoamperometric recordings of KCl-evoked release of DA also showed decreased release of DA in the Str and NAc of the D4R−/− mice.
Careful examination of p62 levels revealed that there was an increase of p62 levels in the STR and SN of parkin −/− mice brain compared to controls (Fig. 1B and 1C), while the other regions of the brain including the HIP, the CB and CTX exhibited no such increase (Fig. S1).
