As seen in Figure 6F, when we compared the levels of FOXM1, CDC42 and RhoB in the sorted ABCG2+ and ABCG2− populations and in the total TamR cells, we consistently observed higher expression of FOXM1 and Rho-GTPase genes in ABCG2+ cells.
Similarly, He et al. (2010) employed GPR125 expression to isolate human spermatogonia and confirmed the expression of α6 integrin, THY-1, GFR α1, and PLZF in the sorted population [ 76].
Moreover, sorting the 1,000,000 iterations of (x_{1}(t)) into sorted data from the smallest to the largest one, the 25,000th and 975,000th value in the sorted data are 28.7396 and 64.0840 respectively.
The chromosome is characterized by a secondary constriction separating the satellite from the short arm and the 5S rDNA locus on the satellite, and its identity in the sorted fraction was confirmed by FISH with probes for 5S rDNA and pSc119.2.
(A) mRNA expression of IL-17A and IL-22 in the sorted F4/80+ macrophages of skin tissue.
The shifts reported in the Results section and shown in Figures 8,10,11 are the means of the resulting difference vectors; the error bars are the empirically calculated 95% confidence intervals (the ranges between the 26th and 975th elements in the sorted difference vectors).
Similarly, equivalent levels of P-glycoprotein and activated AKT were detected in the sorted and unsorted cells (not shown).
The sorting took 60 working days and the average purity in the sorted fractions estimated by FISH using probes for 45S rDNA and pSc200 was 86%.
Chromosome arms 6BS and 6BL were flow-sorted in batches of 59,000 and 49,000, respectively, from approximately 40,000 seeds obtained from 50 dDt6B plants, and the average purity in the sorted fractions, as estimated by FISH, was 91.2 and 92.8% for 6BS and 6BL, respectively.
