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Since substantial heterogeneity exists between the studies for almost all methodological parameters and in the methodological quality, undertaking a meta-analysis was not appropriate.
Due to these differences in the use of SOFA scores and in the methodological approach and quality, results of individual studies are very difficult to compare and meta-analyse.
The TaqMan® Array Human MicroRNA A + B Cards Set version 3.0 (Applied Biosystems/Life Technologies, Carlsbad, CA, USA) was used to quantify expression of 754 miRNAs in the CRC cohort samples and in the methodological samples.
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However, examination of all the above "negative" studies shows that there are bias, confounding factors, and errors in the methodological approach and the data processing and presentation.
HSL planned the design of this study, and participated in the methodological content and writeup of the manuscript.
A concise, integrated comparison follows to highlight the main similarities and differences in the methodological requirements.
The interpretation of any meta-analysis must be made within the context of its limitations, including study selection, publication bias, and variability in the methodological quality of the included studies.
LV, KN, EH and GRJ participated in the methodological and structural design of the study.
MB and AA participated in the methodological discussion and carried out mortality analysis for summer and winter, respectively.
It is evident that there was a wide variation and numerous deficiencies in the methodological quality of the included studies.
MS, HE, EN and SE took part in the methodological decisions, statistical analyses and interpretation of data, commented on the draft, and all authors read and approved the final version.
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