This can be seen all over the world, in the US and UK, and in the mega-constructions of the east namely, China, Dubai, and especially Kazakhstan's Astana.
Francisco Costa was clearly out to make a statement of female strength — in the belted bandeau tops that crisscrossed in back, in the black boots that were a blend of biker and couture, in the mega belt buckles.
The trees were visualised and optimised in the Mega 5.2 program.
Phylogenetic relationships were inferred from the aligned nucleic acid as well as from the amino acid sequences by the neighbour-joining method implemented in the programs DNAdist and Neighbor integrated in the MEGA software package (version 3.1) using the Kimura two-parameter substitution model and a transition/transversion ratio of 10.
Distance and parsimony methods in the MEGA 5 software program were applied to identify the evolutionary scenario for each paralog cluster.
Neighbour joining trees were constructed with the β2 domain of the class II genes and the full amino acid coding sequence of the TAP genes using the Jones-Taylor-Thornton matrix and 1000 bootstraps in the Mega 4.0 software [ 49].
Then, a Y-STR haplotype tree for the 453 male individuals and a mtDNA tree for the 72 individuals were constructed by using the neighbor-joining (NJ) method (Saitou and Nei 1987) in the MEGA 5.0 (Tamura et al. 2011).
Intimations of the trend were evident as early as Airport (1970) and especially in the mega-schlock of The Poseidon Adventure (1972), Earthquake (1973), and The Towering Inferno (1974).
In addition, there was a serious under-representation of French investigators and patients in the mega-trials that become more frequent.
The phylogenetic tree was constructed by the Neighbor Joining algorithm (Saitou and Nei 1987) implemented in the MEGA 6 software package (Tamura et al. 2013).
It is the first Mega Man game for the 16-bit console and the first game in the Mega Man X series, a spin-off of the original Mega Man series that began on the SNES's predecessor, the Nintendo Entertainment System NESS).
