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B-RAF was expressed homogenously in the otic epithelia and in the AVG at HH18 and HH22 (Figure 1 and data not shown), while its expression became more restricted as development proceeded.
Accordingly, Beclin-1 and LC3B are expressed in the otic epithelium and in the AVG, where intense lysosomal activity was particularly observed at the neurogenic zone.
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It is worth noting, that Sorafenib induced less programmed cell death in the AVG than in the otic vesicle epithelium, even though the size of the AVG decreased dramatically (Figure 3 B, arrow in j).
Exposure to Sorafenib caused a dramatic decrease in the area of the AVG, although inhibiting RAF kinase activity did not appear to affect the population of neuronal cells in the AVG, which continued to proliferate and exhibited little apoptosis.
This observation suggested that the RAF-MEK-ERK cascade may be involved in differentiation of neural cells in the AVG, as seen in the differentiation of cortical and dorsal neurons [42], [40].
Surprisingly, the number of clusters chosen by all the combinations of descriptors and datasets was 135, as this gave the largest decrease in the avg-s.
In QuartetM, the "Avg" is replaced by maximum value in all possible scenarios, whereas in QuartetS it is the second minimum value.
VCP expression by Q-RT-PCR analyses demonstrated no statistically significant differences between wild type (AVG ΔCt = 5.65) and R115H knock-in (AVG ΔCt = 5.46) mice with a 95% CI (p = 0.97).
Table 1 summarizes the evaluation of our experiments in: the average (Avg).
Right-click on the AVG icon in the lower-right corner of the desktop.
In the study of Van de Brandhof et al. [ 13] carried out in Holland, the cost per case of AVG was of €77 and in the community study carried out by Hellard et al. [ 7] in Australia €14,27.
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