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The individual amino acids in the sequence and in the alignments are numbered according to the scheme suggested by Ambler [ 10] A systematic comparison of entries of the NCBI peptide database and the TEM mutation table allows to reconcile NCBI peptide database entries which are inconsistent in their mutation profile or their name.
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Sequences and annotation used in the alignments were retrieved using NCSRS software [21].
Pairwise deletion was used to analyze gap and missing data in the alignments.
The frequency of mismatches and gaps in the alignment between contig sequences and the reference genome was determined by BLAST.
Sequences of vaccine A/California/07/2009(H1N1) and A/Brisbane/10/2007(H3N2) strains were included in the alignments and phylogenetic trees.
Additional in-group sequences were obtained from GenBank (Table 1), and included in the alignment.
Signal peptides of the pre-proenzymes were determined and labelled in the alignment.
Gaps and adjacent divergent positions in the alignments were removed.
No indels and stop codons were present in the alignments.
YN and JL participated in the design of the study and in the sequence alignment.
RS participated in the study design and in the sequence alignment, and participated in the writing of the manuscript.
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