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The mixture was painted on Aluminum films (100 μm) and, in order to evaporate the solvent, the electrodes were dried at 120 C for 24 h in vacuum.
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The upper phase which contains almost only gliadin was separated and kept under a fume hood in order to evaporate the ethanol before freeze-drying.
The mixture was stirred and heated in the water bath at 40°C for 2 h in order to evaporate the solvent.
The sample was placed under vacuum for 12 h, in order to evaporate the solvent remaining in the surface.
Then, the tube was heated in a water bath at 75 °C for 5 min in order to evaporate the main portion of the extraction solvents.
Then the seal was broken in order to evaporate the dichloromethane for 4 hours at ambient temperature.
The PTV was operated in the solvent vent mode in order to evaporate the LC effluent.
A backside lift-out was used to reverse the analysis direction in order to evaporate first the 30-nm-wide Al0.05Ga0.95N layer grown below the multilayer stack.
In order to evaporate very gently the remaining solvents and to allow the consolidation of the coating, samples were stored at room conditions for 3 h.
Then, samples were dried and washed with methanol in order to evaporate pyridine and dissolved in acetone (400 μl) and 1 μl was used for GC-MS analysis.
The flask was connected to the vacuum evaporator in order to evaporate solvents.
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