The pellet was resuspended in lysis buffer containing 1% triton and homogenized in a dounce homogenizer.
Before RNA extraction, testes were weighed and homogenized in a Dounce homogenizer (Kontes Co., Vineland, NJ).
3 days after infection, liver, spleen, mesenteric lymph nodes (MLN), Peyer's patches (PP) and kidney were isolated under aseptic conditions, weighed and homogenized in a tissue homogenizer.
Briefly, 6×106 detached hepatocytes were resuspended in SEM buffer (10 mM Hepes-KOH and 250 mM sucrose) containing protease inhibitors as described above and homogenized in a Dounce homogenizer.
Biopsies were teased with sterile surgical blades and homogenized in a sterile homogenizer for 15 minutes.
Renal cortices were weighted, minced, and homogenized in a glass homogenizer.
The pulverized worms were transferred to 6 mL Trizol (Invitrogen, Carlsbad, CA) and homogenized in a dounce homogenizer.
Tissues were cut into small pieces and homogenized in a glass homogenizer in extraction buffer provided in the protein extraction kit (Bio-Rad, Richmond, CA, USA) including 1% protease inhibitors (Bio-Rad) and lysed on ice.
These tissue approaches have primarily relied on a mincing technique, wherein adult tissues are diced with a razor prior to fixation and homogenized in a Dounce homogenizer after fixation [ 21, 22].
The microsomal pellet was resuspended in resuspension buffer (330 mM sorbitol, 5 mM KCl, and 5 mM K2HPO4, pH 7.8) and homogenized in a Teflon homogenizer, and plasma membranes were enriched by phase partition as described previously.
The left lungs of rats were excised, immediately cooled in ice, and homogenized in a Teflon-glass homogenizer.
