Sentence examples for and homogenised in a from inspiring English sources

Between washes thylakoids were resuspended and homogenised in a glass homogeniser and centrifuged for 5 min at 7500×g.

For the DNAse treatment of the chromatin, S2 cells were pelleted, washed in PBS and homogenised in a potter homogeniser using a tight pestle.

Infected monolayers were detached with PBS containing 0.125% trypsin/0.02% EDTA and elementary bodies (EBs) were harvested in DMEM containing 10% FCS at 3,000 × g for 10 min. The infected cell pellet was suspended in PBS:H2O (1:10) and homogenised in a Dounce homogeniser to break open cells and release the EBs.

Approximately 1 g of faeces and 10 g of digesta were suspended in a pre-reduced salt medium and homogenised in a laboratory paddle blender (Seward Stomachers® 80 Biomaster; Lab System) for 2 min.

Tissue samples were thawed at room temperature, 250 mg wet weight of ileum tissue was shredded and then disrupted and homogenised in a buffer containing 4 M guanidine isothiocyanate using a ribolyser (Fast-Prep from Thermo-Hybaid, Runcorn, UK).

The preparation for the Training Set samples at Lab 1 was as follows: a small amount of meat was cut into pieces (∼1 cm) and homogenised in a food processor (Kenwood mini-chopper) for 30 s.

Alternatively, plant material was removed from the co-cultivation plate and homogenised in an autoclaved pestle and mortar, before either being added to molten selection media, or spread over selection media plates.

After treatment, cells were washed twice in PBS and homogenised in an extraction buffer containing 10 mM Tris-HCl, 100 mM NaCl, 10 mM EDTA, 0.5% Nonidet P-40, 0.5% sodium deoxycholate and 0.2% sodium dodecyl sulphate (SDS) at 106 cells/ml and nuclei and large fragments were removed by centrifugation (300×g for 5 minutes).

ScN2a cells were washed twice in PBS and homogenised in an extraction buffer containing 10 mM Tris-HCl, 100 mM NaCl, 10 mM EDTA, 0.5% Nonidet P-40, 0.5% sodium deoxycholate and 0.2% sodium dodecyl sulphate (SDS) at 106 cells/ml.

Tumour samples were thawed and homogenised in an equivalent volume of 50 m M Tris – 150 m M KCl HCl buffer (pH 7.4).

At the end of the treatment, cells were washed twice in PBS and homogenised in an extraction buffer containing 10 mM Tris-HCl, 100 mM NaCl, 10 mM EDTA, 0.5% Nonidet P-40, 0.5% sodium deoxycholate and 0.2% sodium dodecyl sulphate (SDS) at 1 × 106 cells/ml.