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To test this hypothesis, we used A549 cells treated with either control or Nrf2 targeted siRNA and further treated with actinomycin D (ActD).
FA2 with high acid concentration and high TOC wastewater was firstly extracted by amine extractant N7301 to recover FA and further treated by RO system.
Total RNA was extracted using the ZR Fungal/Bacterial RNA MiniPrep Zymo Researchh, Irvine, CA, USA), and further treated with RNase-free DNase I (TaKaRa) to prevent contamination of trace genomic DNA.
The sludge is usually collected and further treated under anaerobic conditions in digester tanks.
(A) A549 Cells were infected with WSN viruses and further treated with different concentrations of L435-3 for 12 h.
Hematite nanoflakes have been synthesized by a simple heat oxide method and further treated by Argon plasmas.
After drying, they are grinded and further treated with formaldehyde to restrict the integration of adsorbent's own colour with the dye tint.
Thereafter, LDPE films were washed with sterile Milli-Q water (Millipore Corporation, Billerica, MA, USA) and further treated with 4 μg ml−1 ethidium bromide for another 15 min.
This LSS was cooled and further treated with 0.5 μl (0.23 amyloglucosidase units AGU) of enzyme dextrozyme DX (Novozyme, Co) at pH 6.5, heated at 60°C, for 24 h and agitated at 300 rpm to produce the hydrolysed sago starch (HSS).
RNA was extracted using TRIzol reagent (Invitrogen) and further treated with DNase I (Invitrogen), and finally purified using the RNeasy kit (Qiagen).
HEK293 cells were incubated for 14 h with nocodazole and further treated for 6 h with either 35.5 µM of CHX or 8 µM of ActD.
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