Supplemental practice was offered on 19 February 2010 to both the two-woman and four-man events out of caution, and further preparation for both events that took place the following week.
The cell suspension was incubated 10 min at 30°C and further preparation was done according to the manufacturer's instructions.
Protein precipitation of plasma with methanol provided sufficiently clean extracts for precise, accurate, and sensitive quantitative analysis of HIV protease inhibitors by MALDI-triple quadrupole MS (see below), and further preparation of the plasma samples with solid phase extraction was therefore not needed.
The isolation and enrichment of mRNA and further preparation including microarray analysis on Affymetrix GeneChip Yeast Genome 2.0 [ 61] were carried out by the Kompetenzzentrum für Fluoreszente Bioanalytik (Regensburg, Germany).
Thirteen preparations were performed with different amounts of DOTATATE (1, 3, 7, 10 and 15 μg) and three further preparations with sodium acetate (130 and 164��mg) instead of HEPES buffer.
Embedding, postfixation, and vibratome sectioning (Leica VT1200 S, Leica Microsystems, Wetzlar, Germany) of adult Wirenia argentea, as well as phalloidin staining of sections and all further preparations, followed the procedures described by Wollesen et al. [ 16].
The heads were deep frozen and stored until further preparations.
After a bit of a break in April preparation began on the series against Pakistan, before the KSL and then further preparation for a tour of the West Indies.
For microtomography experiments, the catalyst bodies were added to a quartz capillary, and no further preparation was carried out.
As shown in Figure 4, 62 food samples in aluminium trays (91%; cakes to be consumed without further preparation and prepared ready-to-serve meals with various ingredients) had an aluminium concentration below 10 mg kg-1.
