The hydrophilic bound precursors were isolated by solid-phase extraction (SPE), enzymatically hydrolyzed to liberate them as the free compounds and further detected by SPME/GC MS.
In a simple target capturing step using aptamer-functionalized magnetic microbeads, signal probes conjugated with streptavidin are released and further detected by a EWA biosensor via a facial dethiobiotin streptavidin recognition.
Mice were euthanized 30 (MDA-MB-231) or 42 (MCF-7) dafterftailtail vein injection, and the lung metastases were examined by live animal Lumina II system (Xenogen IVIS system) and further detected by H&E staining.
Further the WF was resolved as a single band by SDS-PAGE electrophoresis and further detected by Western blotting using a murine antibody to PIF which detects the oligosaccharide chains on the glycoprotein.
Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.
By contrast, DEGs and DEVGs in one pathway can be used as seeds, and further detected their interactive genes in the candidate crosstalking pathways by a random walk restart algorithm [ 28].
In our study, we characterized this phenotypic variation and further detected evidence for local ecotypic adaptation for the CKS ecotype in the form of the longest timescale measurements of establishment and cover.
20 μL of elution buffer was added to elute the purified miRNA in a nuclease-free container and further detected on the 12% denaturing urea poly acrylamide gel electrophoresis (PAGE).
In the present study, we investigated the association of sVCAM-1 with clinical and pathological characteristics in patients with IgAN, and further detected the effect of IgA1 molecules on sVCAM-1 production by cultured endothelial cells in vitro to explore the mechanism of vascular inflammation in IgAN.
For example, Macek et al. (16) profiled 78 phosphorylation sites by high-accuracy mass spectrometry and biochemical enrichment of phosphopeptides from model bacterium Bacillus subtilis in 2007, and further detected 81 phosphorylation sites from the model Gram-positive bacterium Escherichia coli in 2008 177).
