Sentence examples for and freezing in a from inspiring English sources

The sensory epithelia of the inner ear were microdissected and incubated in 25% glycerol for at least 45 min prior to mounting on support planchettes using a yeast in glycerol paste as adhesive, and freezing in a rapidly stirred mixture of propane/isopentane (4:1) chilled in liquid nitrogen.

Nematodes are the dominant soil animals in Antarctic Dry Valleys and are capable of surviving desiccation and freezing in an anhydrobiotic state.

Semen was packaged in 0.25 or 0.5 ml straws and frozen in a programmable freezer using three freezing rates, i.e., −10, −25 or −40 °C/min.

(Mixture can be made 3 to 4 days ahead and frozen in a larger container; stir every 6 to 8 hours to keep mixture loose and evenly frozen).

The eyes were embedded in tissue freezing medium and frozen in a liquid nitrogen-cooled 2-methylbutane bath.

The vessels was then mounted on a holder and frozen in a Leica cryostat (CM 3000, Leica Instruments GmbH, Germany).

Cells were harvested and frozen in a Leica EMPACT high-pressure freezer followed by freeze substitution.

The brains were mounted and frozen in a cryotome and cut into 40  μm sections.

The samples were first centrifuged and frozen in a laboratory in each of the participating countries.

The whole brains were dissected out and frozen in a −70 °C until analysis.

Supernatant was filtered with 0.45-μm filters, aliquoted and frozen in a freezer at −80 °C.