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The analysis of the phase diagram indicates that, except for the minimum-melting point mixture, all mixtures melt and freeze in a temperature range and not at a constant temperature.
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Strain and freeze in an ice-cream machine.
● Grate some cucumber and freeze in an ice-cube tray.
Immediately pipette 50 μl portions into prechilled tubes and freeze in an "ethanol-dry" ice bath.
If you want a healthier ice cream version, use puréed strawberries and bananas and freeze in an ice lolly/icy pole mold.
Semen was packaged in 0.25 or 0.5 ml straws and frozen in a programmable freezer using three freezing rates, i.e., −10, −25 or −40 °C/min.
(Mixture can be made 3 to 4 days ahead and frozen in a larger container; stir every 6 to 8 hours to keep mixture loose and evenly frozen).
The eyes were embedded in tissue freezing medium and frozen in a liquid nitrogen-cooled 2-methylbutane bath.
The vessels was then mounted on a holder and frozen in a Leica cryostat (CM 3000, Leica Instruments GmbH, Germany).
Cells were harvested and frozen in a Leica EMPACT high-pressure freezer followed by freeze substitution.
The brains were mounted and frozen in a cryotome and cut into 40 μm sections.
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