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There was no significant correlation between the preclinical dose (r = 0.323; p = 0.282) or the time between initial dose of intravenous aspirin and first analysis the next morning (r = 0.009; p = 0.96).
Optimization and first analysis can be performed at the light microscopy level prior to more laborious preparation for EM or correlated microscopic analysis.
The day of sample deposition on plate and first analysis is indicated for Le Blanc and Plougasnou samples in Additional File 2. The samples were placed onto standard C. elegans Normal Growth Medium culture plates [ 47] of 90 or 55 mm diameter, previously seeded with an E. coli OP50 lawn in the center.
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A superposition of the thermoelastic fields obtained from the first and third analysis provides the traction-free boundary conditions at the interface of the missing fibers.
Since five mismatches were found between the first and second analysis, the data were randomly analyzed for a third time in order to ensure the authenticity of the data.
The variance of encounter rate for each survey stratum and also for each habitat type within each stratum used in the first and second analysis respectively, was estimated empirically taking each transect line as a sampling unit.
The overall correlations between the first and second analysis by the same observer were good.
MD2 classified 10% of all cases differently between the first and second analysis.
However, in some cases, strong deviations between pairs of results from first and second analysis were also observed.
In the statistical analysis, the p-value was found to be 0.00666, 0.00284 and 0.00555 for the first, second and third analysis, respectively (Table 3).
Similarly, the chi-square values for the first, second and third analysis was found to be 7.36, 8.91 and 7.69, respectively, with one degree of freedom (Table 3).
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