Exact(3)
Then they were ligated successively by overlap-PCR and finally inserted into T-easy vector.
All promoters were cloned by PCR into Gateway compatible Entry clones and finally inserted into the destination lentiviral vector by LR Clonase II recombination according to manufacturer's recommendations (Invitrogen).
Another group, known as RTEs, use a "copy-and-paste" mechanism: an RNA copy of the element is first made by a cellular RNA polymerase, then converted back into DNA by a reverse transcriptase enzyme encoded by the element, and finally inserted into a new genomic location.
Similar(57)
Thus, for predicting the audiovisual quality of IPTV services using the impairment-factor-based model, we first extract parameters from the audio and video packet trace, insert these into (5), (6), (7), and (8), and finally insert the predicted impairment factors into (4).
A single click will automatically download the FNA and PTT files of that genome to the MICAS server, submit the files to IMEx for SSR extraction and finally insert the records into the database.
Throws are quite long, and although Saab finally inserted a little bit more positive engagement feel (there's more certainty when you've actually found a gear) into this tranny, you simply cannot rush shifts.
The nail is finally inserted in the nail fold and kept adherent to the nail bed by a 3-0 external figure U or X suture (Fig. 4a d).
They were 4-5 when Fisher finally inserted Goff into the lineup, and have lost all four games since.
Congress finally inserted a provision in the 2008 farm bill allowing late filers to bring new lawsuits, with their claims to be decided by the same standard of evidence as before.
Resultant transcriptions were finally inserted into a data management software program (NVivo Qualitative Data Analysis Software, 2014) for subsequent analysis.
The implant was finally inserted in the osteotomy site to gradually lift the membrane under total endoscopic guidance from the same lateral sinus trephined hole to ensure again unperforated sinus lining from the lifting procedure during implant insertion.
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