Rough machining of a workpiece in soft stage is followed by a hardening step, usually a batch process, and finalized by a hard machining finishing step.
QPCR cycling protocol was as follows: 3 min initial denaturation at 95°C, followed by 40 cycles at 95°C for 30 sec, 63°C for 30 sec and 72°C for 45 sec, and finalized by a melt-curve analysis (55°C to 95°C, with 0.5°C increments for 10 sec).
The vignettes were assessed by three psychiatrists and finalized by a fourth one.
The survey design was reviewed and finalized by a purpose-assembled working group of UC experts, including SS, JP and EL.
A checklist for each selected PES was prepared based on Hutichson's Clinical Methods (22nd edition) and was peer reviewed and finalized by a core project committee.
Briefly, the standard protocol from Applied Biosystem was used (50°C for 2 min and 95°C for 10 min; then 40 cycles of 95°C for 15 s and 60°C for 1 min) and finalized by a dissociation step (95°C for 15 s, 60°C for 15 s and a ramp rate of 2% to 95°C).
