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For metabolite extraction, 70 mg of frozen powder were weighed and extracted in a pre-chilled methanol: chloroform: water extraction solution (2.5 1 1 v/v).
For fecal antibody analysis, dry fecal pellets were weighed, and extracted in a proportionate amount of extraction buffer (1 ml PBS with 0.1 mg/ml trypsin inhibitor per 100 mg fecal pellet).
Seeds were homogenized using previously cooled mortar and pestle with liquid nitrogen and extracted in a pre-chilled methanol chloroform:water extraction solution (1 2.5 1 v/v) for 30 min at 4°C shaking.
To prepare ELH, dried L. Herba (30 g) was ground into a fine powder, soaked in 300 mL of 70% ethanol, and extracted in a shaking incubator at 40 °C for 24 h.
For DNA fragment run-off sequencing, cleavage bands were cutoff from polyacrylamide gel and extracted in a gel soaking buffer (0.5 M ammonium acetate, 0.1% SDS, 0.1 mM EDTA) at 60 °C overnight and purified by ethanol precipitation.
That data can be tracked in real time and extracted in a variety of ways, including to Excel as a.CSV file, to RSS in the form of email alerts, or for developers as a RESTful API that returns JSON.
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All lymphatic tissue was removed and extracted in an endoscopic bag.
The fresh seeds of two peppers from this batch were divided in two 3-g portions each and extracted in an ultrasonic water bath with either DCM/MeOH (9 1, v/ v) or chloroform.
Cells were cultured for 24 h, washed with PBS, scrapped and extracted in 96% ethanol in a cold methanol bath.
Frozen hearts (30 50 mg) were pulverized using a dismembrator and extracted in 5% trichloroacetic acid.
DNA was also obtained from plants grown at Lawrence Livermore and Los Alamos National Laboratories and was extracted in a similar manner.
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