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The SEM procedures were completed by drying of the material in hexamethyldisilazane (HMDS), followed by mounting and coating with carbon and examining using a ZEISS ULTRA Plus FEG-SEM. 1 ml of blood was drawn in citrate tubes and centrifuged at 1000 rpm for two minutes.
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Fresh D. hybrida labella, petals and sepals were mounted on specimen stubs and examined using a Leo 1455 VP-SEM (Angstrom Scientific Inc., US) and at 400-times magnification.
The grids were dried under natural conditions and examined using a TEM Hitachi H-7500.
Cover slips were mounted onto glass slides and examined using a fluorescence microscope (Leica DMR, ×20).
Ultrathin sections of the specimens were cut and examined using a transmission electron microscope (TEM H-7100, Hitachi High-Tech, Tokyo, Japan).
Thin-section specimens of Bt horizons, and sand and silt fraction grains were prepared and examined using a petrographic microscope.
The specimen was sputter-coated with platinum and examined using a JEOL JSM-6500 TFE-SEM at an accelerating voltage of 5 keV.
The dried samples were sputter-coated with gold/palladium (40/60) and examined using a JEOL JSM-5000LV scanning electron microscope [SEM] (JEOL Ltd., Akishima, Tokyo, Japan).
After immobilization of the samples on the plate, each sample was coated with gold and examined using a scanning electron microscope H-8000, Hitachi, Japan).
After three washes with T-PBS, the cells were mounted with VECTASHIELD Mounting Medium with DAPI Vector Laboratoriess, Burlingame, CA, USA) and examined using a fluorescence microscope (Nikon, Tokyo, Japan).
Finally, the samples were washed thrice with PBS, mounted on glass slides, and examined using a Leica TCS SP2 confocal microscope.
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