Clearly this method works best when at least one co-crystal structure with the natural ligand or an inhibitor is available, but it would also in theory be possible to use an apo structure and examine the interactions made with water.
Nanocomposites based on isotactic polypropylene and montmorillonite were studied, investigating the polymorphism of the polymer, and examining the interaction between polypropylene and silicate.
A specifically formulated nanocomposite based on isotactic poly 1-butene) (poly 1-buteneorillonite was studied, by wide angle X-ray diffraction (WAXD), small angle X-ray scattering (SAXS), transmission electron microscoPB (TEM) and polarized light optical montmorilloniteestigating the polymorphism of the polymer, and examining the interaction betwasn PB and the studiede.
Synergism in the time course was determined using the cumulative cytokine levels after 48 hours by performing a two way ANOVA and examining the interaction term.
Following the suggestions of the reviewers, we performed electrophoretic mobility shift assays and examined the interaction between the recombinant ZNF644 or WIZ proteins and oligonucleotides with consensus sequences.
DOI: http://dx.doi.org/10.7554/eLife.06496.006 To address if the chromatin context affected the interaction between ORCA and G9a, we used the CLTon cells and examined the interaction of YFP-LacI-fused full-length and various truncation mutants of ORCA tethered to the heterochromatic locus, with full-length or truncation mutants of G9a.
To confirm the functional involvement of CD98 phosphorylation in cell-cell interactions, we expressed variant CD98 constructs (Fig. 4A) in CHO cells, which normally do not express CD98, and examined the interactions between these cells and T lymphocytes.
Next, we conducted a separate analysis to control for the potentially confounding effect of body size on sperm number, including pronotum width as a covariate and examined the interactions between body size and treatment.
Correlation analysis will be used to examine the relationships between the different measures of body composition and the MR measures of lipid deposition and to examine the interaction between vitamin D and oxidative capacity by P MRS and cardiorespiratory fitness.
