Bacterial persistence after treatment was confirmed by positive 14C- urea breath test and rapid urease test and histological examination using a modified Giemsa staining method.
In addition they had a focused history and, obstetric examination using a standard clinical record form.
At baseline screening, patients underwent ophthalmoscopy and biomicroscopic examination using a slit-lamp with a fundus contact lens.
As baseline screening, patients underwent ophthalmoscopy and biomicroscopic examination using a slit-lamp with a fundus contact lens.
On the following day, the dogs were anaesthetised for echocardiographic, CMRI and CCT examination using a standard anaesthesia protocol.
All patients received an ultrasound examination of the abdomen, a portal and splenic color and spectral Doppler examination, and left hepatic vein color and spectral Doppler examination using a convex ultrasound-probe (4C1-probe, Siemens-ACUSON S2000, Mountain View, CA, USA).
Other assessments included Von Herrick's peripheral anterior chamber depth, IOP measurement (Goldman applanation tonometry), gonioscopy where possible, and optic disc examination using a 60D lens to assess VCDR, cup disc asymmetry and the presence of splinter haemorrhages or a notch.
They were finally counterstained with DAPI and mounted for examination using a Nikon confocal microscope (Eclipse Ti, Nikon, Japan).
Each subject underwent a comprehensive ophthalmic examination, including best corrected visual acuity testing, slit-lamp biomicroscopy, intraocular pressure (IOP) measurement with Goldmann applanation tonometry, gonioscopy and dilated funduscopic examination using a superfield fundus lens; a review of the medical history was also conducted.
Tissue sections of 4 μm were cut and stained with haematoxylin and eosin, followed by examination using a Leica DM-RXA.
