First, sampling of integration sites was limited, and examination of phylogenetic trees suggests additional proliferating cell populations for which integration sites were not derived (Fig. 2 and figs. S2 and S3).
This left us with 496 SNPs for which we sought to detect the presence of the AA, AB, BB, A, and B chromosomes in our sample set, through a combination of phasing and examination of individual genotypes (see Additional data file 4).
Although in Additional file 1: Figure S1 there appears to be a partial reduction in IHC signal due to the non-phosphorylated peptide (+mTOR) and an irrelevant p-peptide (+pS118), close examination of Additional file 1: Figure S1C and 1D and comparison to Additional file 1: Figure S1A shows that the dynamic signal intensity range among positive cells is similar.
While examination of additional critical periods and in depth mechanistic studies is warranted, the results of this investigation clearly show the dose-dependent teratogenicity of this SCB.
Quantitative modeling of soil C and N as in our study supports examination of additional ecosystem properties at fine spatial scales.
Examination of additional CpG sites and asthma genes may help elucidate the time course of epigenetic change of other genes important to airway inflammation.
After detection of changes in fetal hematopoiesis indicative of infection, examination of additional specimens of placenta and membranes will document the diagnosis of chorioamnionitis.
In future, in depth examination of additional human cell lines and tissues, representing a wide range of physiological and pathological states will surely offer new insights into the p63 family of factors and their broad transcriptional network.
Examination of additional breast cancer databases and prospective evaluation of clinically annotated breast tumor biopsies will be necessary to further define how the combination of low BECN1 expression and WNT pathway activation affects prognosis within the different breast cancer subtypes.
The difficulty in assigning pharmacological activities to specific sequence variations is immediately apparent upon a cursory examination of Additional file 11: Figure S4 and Additional file 12: Figure S5.
