Sentence examples for and enumerated using a from inspiring English sources

Spots were developed with tetramethylbenzidine (TMB) substrate and enumerated using a dissecting microscope.

The particles delivered were collected and enumerated using a PS lased based particle counter.

After washing with tap water and 1 h incubation with streptavidin-AP conjugate (Dako) at room temperature spots were developed using alkaline phosphatase substrate BCIP/NBT (Sigma) and enumerated using a dissecting microscope.

Cells expressing high levels of ALDH become brightly fluorescent (ALDHbr) and can be identified and enumerated using a standard flow cytometer or isolated by cell sorting for further purification and characterization.

After 120 hours, harvested cells were subject to heat and sonic disruption, and enumerated using a hemacytometer.

The cell monolayers were harvested by trypsinization and single-cell suspensions were prepared and enumerated using a Z1 Coulter counter (Beckman Coulter, Fullerton, CA).

Spots were visualized using an alkaline phosphatase conjugate substrate kit (Biorad, Hercules, CA, USA) and enumerated using an automated ELISpot reader.

Noninvasive cells were removed from the upper chamber with a cotton swab, and migrating cells adhering to the underside of the filter were fixed, stained with May Grumwald and Giemsa and enumerated using an optical microscope.

At the end of the incubation period, the cells were harvested by trypsinisation and enumerated using an automated particle counter.

The spots were developed with NBT/BCIP (Thermo Fisher Scientific, Waltham, MA, USA) and enumerated using an automated ELISPOT reader and software (AID Diagnostika, Strassberg, Germany).

Single cell suspensions of the thymus, spleen and bone marrow were prepared and enumerated using an automated CASY counter (Scharfe, Krämerstraβe, Reutlinger, Germany).