Sentence examples for and enriched using a from inspiring English sources
Size-selected fragments were bisulfite-treated using an EZ-DNA methylation kit (Zymo Research) and enriched using a MethyMiner methylated DNA enrichment kit (Invitrogen).
After 24 h, fluorescent cells were resuspended and enriched using a MoFlo (Beckman Coulter) according to the instructions of the manufacturer.
CTCs were isolated and enriched using a slide-based assay using ferrofluid linked to antibody to EpCAM (epithelial adhesion molecule) and identified by phenotyping using antibodies against a pan-cytokeratin (C11; Sigma, Natick, MA, USA) and HER2 (Her 81; provided by Dr J Uhr, University of Texas Southwestern Medical School, Dallas, TX, USA).
> -wrap-foot> We present a method where multiple DNA samples are first indexed and combined into one sequencing library and then target enriched using a single reaction.
Seven days following injection, mice were sacrificed and lung and spleen cells were enriched using a homogenizer.
Seven days following the 2nd transfer, mice were sacrificed and lung and spleen cells were enriched using a homogenizer.
(40) Phosphorylated tyrosine containing proteins and peptides can be enriched using a specific antibody directed against phosphotyrosines.
PBMC were thawed, and CD8+ T cells were enriched using a MiniMACS device (Miltenyi Biotec, Bergisch Gladbach, Germany) resulting in >90% CD3pos cellss cells.
The tissues were solublized, and the FLAG-tagged SOD1 and cross-linking proteins were selectively enriched using a FLAG affinity purification system.
Total amounts of 32 ug of DNA NA12716 (c = 265 ng/uL in TE) and 30 ug of DNA NA12760 (c = 150 ng/uL in TE), both with A/A280 ≥1.8 and A/A≥1.9 (measured by Nanodrop) were enriched using a customer designed 385K 1-plex sequence capture array following the manufacturer's instructions (Roche NimbleGen, Madison, WI).
Thus, retinal photoreceptors derived from iPS cells and H-1 hES cells can be enriched using a combination of IRBP-GFP lentivirus followed by FACS.
