Sentence examples for and efficient amplification of from inspiring English sources

aureus) genes, and in the replication origin sequence (oriC, Salmonella spp .. Melting-curve analysis using a SYBR Green I RTi-PCR approach showed characteristic Tm values demonstrating the specific and efficient amplification of the three fragments.

For successful microarray analysis unbiased and efficient amplification of this RNA is also required.

These were also associated with mean β-actin 3'/5' ratios of 2.61±1.07 and GAPDH 3'/5' ratios of 1.59±1.21 suggesting high quality and efficient amplification of RNA (Table 6).

Here we provide two approaches for the rapid and efficient amplification of amphibian mitogenomes from microgram quantities of tissue.

To ensure the accurate and efficient amplification of methylated TGFBI DNA by the methylation-specific primers, we optimized the annealing temperature for these primers using 293T and H522 tumor cells as positive controls, and normal human bronchial epithelial (NHBE) cells and human mammary epithelial cells (HMEC) as negative controls.

Therefore, the careful optimization of reaction components and conditions will ultimately result in more reproducible and efficient amplification.

In search for a PCR mix compatible with all the DNA dyes, and suitable for efficient amplification of difficult-to-amplify DNA templates, such as those in whole blood, of medium size and/or GC-rich, we found excellent performance of a PCR mix supplemented with 1 M 1,2-propanediol and 0.2 M trehalose (PT enhancer).

β-actin and GAPDH 3'/5' ratios (<2.57 and <1.07 respectively) indicate efficient amplification of the RNA template at all levels of input with no observed 3' over-bias (Table 2).

Humanized transgenic mouse (129MM) brain substrate similarly showed efficient amplification of vCJD and C-type BSE and readily detectable amplification of CWD PrPres using 3F4.

Primers and fluorescent markers were designed to allow efficient amplification of DNA extracted from FFPE tumours and resolution of different amplified products.

Our results suggest that certain avian hosts within Mexico are competent for efficient amplification of both northern and southern WNV strains and that both strains likely contribute to bird deaths.