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The gels spots were destained with 50 μ1 50%.acetonitrile (ACN) in 25 mM NH4HCO3 and dried using a speed vacuum for 10-15 min. To the dried spots, 10 mM DTT dissolved in 100 mM NH4HCO3 was added and incubated for 1 hour at 56°C.
Samples were then washed with pure D2O to remove salts and dried using a slow stream of N2 gas.
The dispersion was allowed to cover the substrate completely and dried using a spin-coating technique.
30 ml yeast cultures were collected separately and dried using a freezer dryer.
The collected yeast cells were washed once with distilled water and dried using a freezer dryer (Alpha 2 4 LSC, Christ GmbH).
The glass lens and PDMS block were cleaned using an ultrasonic cleaner with ethanol and dried using a nitrogen spray gun.
The sample was then removed and vigorously washed several times using pure water to remove any precipitate on the surface and dried using a flow of nitrogen gas.
The prepared nanocomposite was washed with DI, following which the Fe3O4-PNIPAAm-2 was separated out by centrifugation (12,000 rpm for 30 min) and dried using a rotary evaporator (25 mbar at 40 °C).
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The arrays were washed, as recommended by manufacturer, and dried using an Array-It mini-centrifuge.
To ensure the tubing was secure an additional cyanoacrylate layer was applied around the tube and dried using an accelerator fluid (Zip kicker, Pacer).
Following pre-hybridization slides were rinsed thoroughly with sterile distilled, deionized water (DDW), and dried using an ArrayIt microarray high-speed centrifuge (TeleChem International, Sunnyvale, CA).
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