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Visualization of PCR product was done by subjecting 10 μl of amplified reaction mixture to electrophoresis on a 2% agarose gel incorporating 5 μg ml of ethidium bromide in 1× Tris-Borate buffer (pH 8.2 8.6) and documented on gel documentation system (Vilber Lourmat, France).
PCR products were resolved by agarose gel (2%) electrophoresis with ethidium bromide and documented on a UVP Gel Documentation system (UVP, Cambridge, UK); 398- and 424-bp fragments represented spliced and unspliced XBP-1 transcripts, respectively.
The contestants are not allowed to be good friends, she attends every meal as a formal adjudicator and the entire event is carefully photographed and documented on her blog.
She may not sing Isolde again, she said, or another highly challenging Wagner role, Brünnhilde, which she performed in the Metropolitan Opera's high-stakes production of the "Ring" cycle, directed by Robert Lepage and documented on a set of DVDs.
(Nate Chinen) Lotte Anker (Wednesday) Ms. Anker, a Danish saxophonist drawn to abrasive texture and searching polytonality, appears in a trio well-traveled in Europe and documented on several albums.
2. The land boundary shall be demarcated, under a joint boundary demarcation procedure, by boundary pillars which will be jointly located, erected, measured and documented on the basis of the boundary shown in the 1 20,000 orthophoto maps referred to in Article 2-C(1) above.
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"There is more to explore and document on the East Coast".
VS and its course was observed and documented based on the following criteria.
His drive and commentary were documented on Web video.
This is acceptable and should be documented on the case report form.
Procedures were video-recorded and representative findings documented on high resolution images using software program.
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