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The solid freeze-dried residue was suspended in water and dissolved with a 0.50 M NaOH solution by automatically titrating under N2 to pH 7.2 (VIT 909 21 Videotitrator, Radiometer, Copenhagen) until pH remained constant for 120 min.
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A desired amount of morphine HCl (e.g. 6 mg for routine calibration; 4 mg for performance checking) was weighed and dissolved with methanol in a 10 mL volumetric flask.
OY, OY-A and OY-B powder was weighed and dissolved with deionized water at a concentration of 20 mg/mL.
CY and FCY powder were weighed and dissolved with deionized water at a concentration of 20 mg/mL.
For dose-response assays, the CDP mix was evaporated to dryness, weighed out, and dissolved with DMSO to prepare a 100 mg/mL concentration as stock solution.
The 95% ethanol eluent was condensed and dissolved with methanol and then placed in a 2-mL flask with stopper, with a methanol-metered volume.
Finally, the extractions were dried in a SpeedVac and dissolved with 10 μl 0.1% formic acid.
The extract obtained was used as an original extract, and dissolved with pure water when necessary.
The coagulum was readily collected from the solution and then dissolved with a small amount of N,N-dimethylformamide for the introduction to a graphite furnace of atomic absorption spectrometer.
Structurally, it has an almost 19th-century delicacy, with quasi-formal groupings crystallising and dissolving with the kaleidoscopic variety of a Romantic-era dream sequence.
Of children whose pain dissolved with a cannabis candy.
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