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After the incubation, cells were washed completely with Hank's balanced salt solution (HBSS) four times and dissociated with a fire-polished glass pipette in dissociation medium (HBSS, 0.1% BSA and 8 mM MgCl2), pelleted by centrifugation at 4000 g for 4 min at room temperature (RT), dissociated in starter medium (DMEM containing 10% FCS) and plated on coverslips.
Briefly, testes were dissected, the albuginea layer removed, tubules cut into small pieces and dissociated with a pipet in PBS.
Briefly, midbrain tissues were dissected from embryonic day 13-14 Sprague-Dawley rand andissociateded with a mild mechanical trituration.
Mouse cortices were dissected from embryonic day (E) 13.5 to E 16.5 animals, trypsinized with TrypLE express at 37 °C for 10 min, and dissociated with a fire-polished Pasteur pipet in plating medium (Neurobasal medium with 5% fetal bovine serum, Glutamax, B27 (Invitrogen), and Antibiotic-Antimycotic (Invitrogen)).
Similar(56)
Similarly, in type II (dissociated) fatty acid synthases and in many other enzymes that react with ACP-bound groups, the ACP must be able to associate and dissociate with a variety of protein partners and must be capable of exposing the fatty acyl chain to thioesterases and other enzymes.
Resting and stimulated EC were harvested and dissociated with Accutase™ (Sigma-Aldrich), a mixture of proteolytic and collagenolytic enzymes from an invertebrate species.
Mesencephalic tissues were isolated and dissociated with gentle mechanical trituration, followed by an enzymatic digestion in Trypsin/ethylenediaminetetraacetic acid diluted in warm HBSS w/o Ca2+ and Mg2+ (1:1) for 20 min at 37°C.
H1 and H9 cells were harvested and dissociated with 0.25% trypsin/EDTA (Invitrogen).
Briefly, the hippocampi were dissected from E18 BL21 mice and dissociated with 0.25% trypsin and trituration.
ESCs were split between 1 4 and 1 8 every 2 3 days and dissociated with Accutase (Sigma).
Briefly, TA muscles from 1-month-old mice were dissected and dissociated with collagenase (Roche).
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