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In this method, proteins are digested into peptides, which are then ionized and dissociated in a mass spectrometer.
The injection of the Rous sarcoma virus (RSV) into the wings of chickens also produces large and lethal malignant tumours but injecting RSV into the wings of early chick embryos did not induce tumours and the embryos continued to develop normally; but if those embryonic chicken wings were removed and dissociated in a culture dish then they rapidly developed a transformed phenotype [ 45].
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All 4 circuits were replicated and dissociated in an independent data set (n = 40).
Whether HSP47 binds nascent peptides, monomers, unhydroxylated trimers or hydroxylated triple helices is debated (46– 49); however, it appears to transiently associate with Y-position arginine motifs (i.e. Gly-X-Arg) (47, 49, 50) and dissociate in a pH-dependent manner after trafficking to the more acidic cis-Golgi (48).
RNA granules are dynamic and can be reversibly associated and dissociated in vivo as well as in a cell-free system.
Thymuses were removed from 6 8 wk-old mice and dissociated in RPMI 1640 medium.
Animal caps were excised from stage 8 embryos and dissociated in Ca2+ Mg2+ free MBS.
For passaging, neurospheres were harvested and dissociated in 0.05% trypsin (Gibco) followed by addition of trypsin inhibitor.
Approximately 3 4 months post-induction, tumor tissue was harvested and dissociated in 0.05% Trypsin-EDTA (Life Technologies).
Ventricles were trisected, pooled, and cardiomyocytes dissociated in a solution of collagenase and pancreatin.
Briefly, cortices from rats at embryonic day 15.5 were dissected and mechanically dissociated in a modified serum-free, HEPES-free N2-supplemented DMEM/F12 medium (Invitrogen, Carlsbad, CA, USA).
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