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Mice were euthanized by CO2 inhalation, and left lung lobes were aseptically removed and disrupted in a tissue homogenizer as previously described [44].
Cell pellets were resuspended in buffer (10 mM TRIS, pH 7.5, 250 mM sucrose, 2 mM dithiothreitol (DTT) and 1 tablet Roche Complete™) and disrupted in a Parr Bomb (Parr Instruments Deutschland GmbH, Frankfurt/Main, Germany).
In total, 50 100 mg of infected plant tissue from herbarium voucher specimens (Table 2) were taken and disrupted in a mixer mill (MM2, Retsch, Germany), using six iron balls of 3 mm diameter per sample.
In brief, ~1.5*10 cells were harvested and disrupted in a beadmill.
Yeast pellets from 2 ml of exponential phase cell culture were mixed with 0.2 ml of glass beads (diameter, 0.45 mm) and disrupted in a Fastprep FP120 (Bio-101, Thermo Savant, California, USA) by a 45-s burst at speed 6.
Cells were harvested and disrupted in a radioimmunoprecipitation assay (RIPA) lysis buffer buffer (50 mM Tris HCl, pH 8.0, 150 mM NaCl, 0.1% SDS, 1% NP-40, 0.25% sodium deoxycholate and 1 mM EDTA with protease inhibitor cocktail, 1 mM NaF and 1 mM Na3VO4).
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For the analysis of protein levels, cells were harvested and disrupted in an appropriated lysis buffer.
Cells were harvested, resuspended in buffer A (50 mM Tris/HCl pH 7.8, and 200 mM NaCl), and disrupted in an Emulsiflex.
Judges' comment: In assembling the world's biggest database of spas, Wahanda is taking on the biggest players and disrupting in a huge niche.
"I think there's something about having that image or having the object and putting it out there that confronts people, that connects and disrupts in a way that's very different than words".
To detect p53 activation in vivo, tumours were harvested and disrupted in RIPA buffer containing a protease inhibitor mixture (Sigma).
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